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Factors Affecting The Rate Of Enzymes Activity Biology Essay Factors Affecting The Rate Of Enzymes Activity Biology Essay
Published: Mon, 5 Dec 2016 Enzymes are catalysts made within the human body. Catalysts naturally, lower the activation energy required for reactions. The lower the activation energy is, the faster the rate of reaction is, and therefore enzymes speed up reactions in the body by lowering the activation energy required.

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Also, the fact that we have to reset it to 0 at the start, whilst the reactants are already reacting, provides unreliable timing, and therefore unreliable results. This is why my chosen method for measuring the rate of reaction is the gas syringe method. In the science industry, a human reliant factor would be extremely impractical because of the lack of accuracy.

As the reaction takes place, the gas products move towards the measuring cylinder, where the water is replaced with the gas molecules. I therefore reject this method of measurement. I must also make sure that the temperature of the water and the trypsin thermometers etc, the readings are taken with the graduated markings at eye level, so as to minimise the risk of inaccurate readings.

Reactions that happen slowly have a low rate of reaction. This method produces rather reliable and scientific results, if, of course, it is set up properly to measure. The only method left to use is the gas syringe, which appeared to be the most reliable and feasible to set up, as well as being a scientific way of measuring.

Another reason could be that the time at which the stopwatch was started meant that it was more than 30 seconds of juice obtained. It is also time consuming, which isnt practical. The measurement of the experiment would be difficult.

I made black coffee for each individual(no milk). If the temperature is not kept constant anomalies will result. For test 2 i made sure that they didnt have any caffeine taken for at least a day.

At the end of the experiment, (i. An investigation to look at the effect of pectinase on other fruits such as plums or grapes or investigate changes in concentration of other enzymes such amylase or catalase. The fact that the reactants had already started to react whilst the timer hadnt even been set yet due to the fact the top pan balance had to be reset was an extremely unreliable factor. When the x is no longer visible, the timer is stopped and the timing is measured. This would have provided more data and therefore the graphs and rate of reaction would be more accurate.


Gram Positive and Gram Negative Bacteria Experiment


The aim of this experiment was to differentiate between Gram negative and Gram positive bacteria by Gram staining method. In addition, this experiment allowed us to visualise the morphology and arrangement of the bacterial cell. In this experiment, two different staining methods were employed, the ...

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Chemistry Rate of Reaction Coursework for Calcium Carbonate ...
Investing Rate of Reaction. I am going to investigate the rate of reaction between two substances: calcium carbonate and hydrochloric acid. Rate of reaction measures the speed at which a reaction takes place.
Catalase Coursework A website mainly aimed at students studying A-Level Biology - covers genetics, cells, biochemistry, enzymes, ecology, environment, photosynthesis, respiration, heart. This is why my chosen method for measuring the rate of reaction is the gas syringe method. It is dependent on the equipment, which also widens more room for error in the equipment used. The tubing should be positioned well on the rear end of the gas syringe. Investigate the rate of reaction between hydrochloric acid and calcium carbonate. I think the speed of the reaction will considerably change from the temperatures 200c to 600c (the rate of reaction will increase). Products included in the brand claim to do things like detoxify the genes, enhance’s the body’s defense system, Also. I must also make sure that the temperature of the water and the trypsin thermometers etc, the readings are taken with the graduated markings at eye level, so as to minimise the risk of inaccurate readings. Basic plan this experiment will be set up as shown below in the diagram of experiment.
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    The available methods left are the gas syringe method, and the displacement of water, which compared to the others, turned out to be the most practical, reliable and innovative ways of measurement. Created by teachers, our study guides highlight the really important stuff you need to know. This method is easy to set up and reset. These investigations would extend the enquiry of the enzyme pectinase and also into the substrate it is having an effect on. The stopwatch was started after all the contents of the test tube were emptied, which took a few seconds and during this time juice was filtering through.

    Every enzyme has an optimum ph. This whole method (apart from the flask and part of the tubing) should be carried out in a water tray, full of water. Explosions have a high rate of reaction. The two most often used meat tenderizing enzymes are papain and bromelain. .

    This method works by measuring the loss of mass as a as forms. In the science industry, a human reliant factor would be extremely impractical because of the lack of accuracy. In other terms, theres less dependence on human ability, which can easily cause an error. It is also time consuming, which isnt practical. After trialling all of the methods for measuring the rate of reaction, i have formed a clear view of the reliability of each method, and the practicality. When the x is no longer visible, the timer is stopped and the timing is measured. I therefore reject this method of measurement. The fact that the reactants had already started to react whilst the timer hadnt even been set yet due to the fact the top pan balance had to be reset was an extremely unreliable factor. Another reason could be that the time at which the stopwatch was started meant that it was more than 30 seconds of juice obtained. This is measured by                                                                      time taken different reactions can happen at different rates.

    The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative, aerobic or facultative anaerobic rod which produces gas from lactose within 48 hours.

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    A website mainly aimed at students studying A-Level Biology - covers genetics, cells, biochemistry, enzymes, ecology, environment, photosynthesis, respiration, heart ...
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    If i increase the temperature of the water the rate of the reaction would also be quicker because there would be more energy in the molecules and there would be more collisions, therefore they will react with each other quicker. Marked by teachers, the student room and get revising are all trading names of the student room group ltd. The timer is started straight after. The simplicity of this method does extent the time availability, which allows more results to be carried out. This method works by measuring the loss of mass as a as forms.

    The reactants are mixed in the conical flask. However, unlike timing and observation, this method is far more accurate, so a result or two less would not be much trouble Buy now Catalase Coursework

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    The only method left to use is the gas syringe, which appeared to be the most reliable and feasible to set up, as well as being a scientific way of measuring. Introduction enzymes are proteins that speed up and control certain reactions. The setting up of this method does not require so much hassle or attention, yet the procedure of measurement provides accurate results. It is supported by clear figures that add to the textual information provided. Using a slightly bigger test tube or even a boiling tube could do this.

    This method did not prove such reliable measurements and results seeing as the observation would have to be done using human activity, especially eyes instead of a reliable apparatus Catalase Coursework Buy now

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    It is two stars as it only covers the initial research into the appropriate method. The tubing should be positioned well on the rear end of the gas syringe. A reaction must be at a convenient speed to measure, because a fast rate of reaction can be hazardous, and a very slow rate of reaction is not practical. This method of measurement in products is extremely easy to measure and set up. The easy set up allows more time for more results which allow a fairer mean.

    The flask is put upon the top pan balance, and it is reset to 0, so that the weight of the flask is not included (only the weight of reactants). This would have provided more data and therefore the graphs and rate of reaction would be more accurate Buy Catalase Coursework at a discount

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    This pressure is realised by a push forced onto the plunge inside of the gas syringe, which shows the exact ml of product produced to be visible by the scale of measurements on the outside of the gas syringe. The increased number of equipment used provides a more equipment dependent method, which, if one part of the equipment fails, so will the whole method. For example, rusting has a low rate of reaction. Activation energy shown on the energy level diagrams below. Reactions that happen slowly have a low rate of reaction.

    They work by the substrate molecule binding with the enzyme to form an enzyme-substrate complex. Explosions have a high rate of reaction. However this could only be done with more time in which to carry out the experiment Buy Online Catalase Coursework

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    This is a ph level that the enzyme works fastest at. The flask is put upon the top pan balance, and it is reset to 0, so that the weight of the flask is not included (only the weight of reactants). The tertiary structure of the protein gives a certain shape to the enzyme and active site and therefore means that only certain shape substrate molecules will fit. Place calcium carbonate in conical flask, and quickly pour in hydrochloric acid. It is then carefully chopped up into small pieces using a knife and a tile.

    This final volume will not be needed in order to calculate the initial rate of reaction, but is worth recording in order to see how much juice the apple produces and if the method is successful in producing apple juice Buy Catalase Coursework Online at a discount

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    This method is easy to set up and reset. There is even more collagen in the skin, bones, teeth, blood vessels, eye, heart, and, in fact, pretty much in all parts of the body. Every enzyme has an optimum ph. Investigate the rate of reaction between hydrochloric acid and calcium carbonate. This method of measurement in products is extremely easy to measure and set up.

    The upwards arrow up represents this minimum energy needed to break bonds to initiate the reaction. The tubing should be positioned well on the rear end of the gas syringe. Each enzyme molecule has an active site, where the substrate molecules become attached. Put on lab coat and goggles for protection against dangerous chemicals Catalase Coursework For Sale

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    Every enzyme has an optimum ph. They also increase the viscosity of the juice, making it difficult to obtain optimal yields. The pressure in the gas syringe is built up because of the air-quality of the gas syringe, where it is air tight. Compared to, for example, timing and observation, this method is harder to set up and takes away time which couldve been used to record more results, therefore providing more reliability. This method works by measuring the loss of mass as a as forms.

    This is why i reject these two methods. For fruit manufacturers, the addition of industrial pectinase between mashing and pressing causes complete de-pectination an investigation into the inhibiting effect of tomato juice on the germination of cress For Sale Catalase Coursework

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    I waited 10 minutes and then gave them the test. Investigation of the effect of adding different concentrations of nacl to an enzyme-substrate (amylase-starch). It is supported by clear figures that add to the textual information provided. The rubber bung should be placed extremely fast after this process, as well as the starting of the timer. To make the experiment a fair test i am going to make sure that volumes of trypsin and ph buffer are the same in each experiment and the size of the photographic film is the same.

    At the end of the experiment, (i. It is possible to go on and look at the effect of temperature on pectinase and also substrate concentration, however the latter would be fairly difficult to measure Sale Catalase Coursework

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